Journal:

Article Title: Dissemination of Streptococcus pneumoniae Clone Colombia 5 -19 in Latin America

doi: 10.1128/JCM.40.11.3942-3950.2002

Figure Lengend Snippet: PFGE subtype patterns of S. pneumoniae serotype 5 invasive isolates. Chromosomal DNA fragments were digested with SmaI. Lanes 1, 15, and 28 (counting from the left) correspond to Lambda ladder; lanes 2 and 27 contain strain R6. Lanes 3 to 10 and 26 show PFGE pattern A: Col 7, Col E330, Col E390, Bra 102, Bra 291, Gua 168, Gua811, Mex 49, and Col 133. Lanes 11 and 12 show PFGE pattern A1: Col E68 and Col 136. Lanes 13 and 14 show PFGE pattern A3: Col E72 and Mex 146. lanes 16 to 19 show PFGE pattern A5: Arg 1061, Arg 1105, Uru 1215, and Mex 110. Lanes 20 to 23 show PFGE pattern A6: Uru 216, Arg 1108, Col 72, and Bra 71. Lanes 24 and 25 show PFGE pattern A12: Uru 332 and Uru 112.

Article Snippet: The molecular weight marker used was the lambda ladder (New England Biolabs, Beverly, Mass.) and the R6 strain.

Techniques:

Journal:

Article Title: Molecular Characterization of Multidrug Resistance in Streptococcus mitis

doi:

Figure Lengend Snippet: Results of SmaI PFGE (A) and HinfI digestion of 250-bp gyrA PCR amplicons (B) of the 14 S. mitis isolates. Lanes 1 and 2, S. mitis strains isolated from the case patient in April and May 1996, respectively; lanes 3 through 14, 12 noncase S. mitis isolates. Lanes M, Lamba Ladder PFG marker (New England BioLabs, Beverly, Mass.) (A) and the 100-bp DNA ladder (Gibco BRL, Gaithersburg, Md.) (B).

Article Snippet: Lanes M, Lamba Ladder PFG marker (New England BioLabs, Beverly, Mass.) (A) and the 100-bp DNA ladder (Gibco BRL, Gaithersburg, Md.) (B). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Isolate MIC (μg/ml) a of: Molecular characterization by: CIP ERY CLI TET PEN CTX CRO IMP VAN PCR tet (M) d hybridization gyrA b mef c ermB c Case April 1996 64 >32 >16 >16 1 0.12 0.5 1 1 + − + + May 1996 64 >32 >16 >16 1 0.5 1 0.5 1 + − + + Noncase 1 0.5 >8 >8 ≤0.5 0.5 1 1 0.06 ≤0.5 − − + − 2 0.5 ≤0.5 ≤0.5 >4 0.06 0.06 0.06 0.06 ≤0.5 − − − + 3 2 ≤0.5 ≤0.5 >4 0.03 0.06 0.06 0.03 ≤0.5 − − − + 4 2 ≤0.5 ≤0.5 ≤0.5 0.5 0.25 0.25 0.06 ≤0.5 − − − − 5 2 >8 >8 ≤0.5 0.25 0.5 0.25 0.06 ≤0.5 − − + − 6 0.5 1 ≤0.5 ≤0.5 1 0.5 0.5 0.25 ≤0.5 − + − − 7 64 >8 >8 >4 0.03 0.06 0.06 0.06 ≤0.5 + − + + 8 0.5 2 ≤0.5 ≤0.5 0.03 0.06 0.06 0.03 ≤0.5 − + − − 9 4 >8 >8 >4 0.25 1 0.5 0.06 ≤0.5 − − + + 10 1 ≤0.5 ≤0.5 ≤0.5 0.03 ≤0.015 ≤0.015 0.03 ≤0.5 − − − − 11 0.5 >8 >8 >4 >8 >4 >4 2 ≤0.5 − − + + 12 0.5 2 ≤0.5 ≤0.5 4 2 2 1 ≤0.5 − + − − Open in a separate window a CIP, ciprofloxacin; ERY, erythromycin; CLI, clindamycin; TET, tetracycline; PEN, penicillin; CTX, cefotaxime; CRO, ceftriaxone; IMP, imipenem; VAN, vancomycin. b gyrA PCR amplicons (250 bp) were digested by Hin fI.

Techniques: Isolation, Marker